图1所示。(A)慢性酒精蒸气暴露导致血液酒精浓度稳定和躯体戒断评分显著增加的方案。禁欲3周后，分批只动物被用于脊柱密度分析、注意力转移测试(注意集转移测试)或慢病毒边缘下mGluR2抢救。(B)皮肤助理试验方案，大鼠必须根据嗅觉或触觉线索/尺寸做出连续的区别性选择。与对照大鼠(n = 15，白色圆圈)相比，酒精依赖[后依赖(PD)， n = 14，黑色方框]大鼠在IDS和EDS子任务中需要更多的试验来评判。(C)延迟贴现任务(DDT)方案。下图表示对高奖励的偏好与高奖励在时间上延迟的百分比。与对照组相比，酒精依赖大鼠的曲线更陡(平均值±SEM)。(D) P大鼠过早终止密码子基因组定位示意图(50)。(E) Alcohol-naïve印第安纳州P大鼠(n = 12，黑色三角形)与印第安纳州NP大鼠(n = 12，白色三角形)在IDS/EDS任务上没有差异。 (F) Schematic representation of lentiviral constructs. Lenti-mGluR2 expresses Grm2 and cop green fluorescent protein (copGFP) under control of elongation factor 1-alpha (EF1α) promoter. Lenti-control only expresses copGFP under EF1α control. ENV; Epstein-Barr virus derived vector, cPPT; central polypurine tract, WPRE; Woodchuck posttranscriptional regulatory element. (G) Injection placements are represented by black circles. Injection sites were verified within the infralimbic cortex from +3.2 to +2.2 mm anterior to bregma (85). (H) ASST performance of PD rats injected with either lenti-mGluR2 (n = 16, red squares) or lenti-control (n = 16, black squares) into the infralimbic cortex. In the EDS component of the task, no difference was observed between control and virally infected PD rats. However, in the IDS component, lenti-mGluR2–injected animals overall needed significantly fewer trials to criterion. IDS, intradimensional shift; EDS, extradimensional shift; *P < 0.05, **P < 0.01, and ***P < 0.001. Credit: DOI: 10.1126/sciadv.abh2399
