蛋白质组分析健康和非小细胞肺癌科目使用5 NP等离子工作流。答:这个概念验证proteoform识别研究的概述。血浆样本收集的健康(蓝色),早期非小细胞肺癌(NSCLC;黄色)、晚期NSCLC(橙色)和风险(绿色)对象(样本集合)。血浆蛋白质组分析的每一个科目,其中包括蛋白质提取,蛋白质发现使用NP-based Proteograph平台,然后DIA蛋白质/肽识别和量化使用质/ MS和搜索算法(蛋白质组分析)。Proteoforms被发现使用一个不和谐的肽强度搜索,其中包括研究已知蛋白肽映射编码亚型和使用微分丰富发现蛋白亚型。在一起,这些标识proteoforms代表一个扩大血浆蛋白质组数据库没有捕捉到标准MS-based或有针对性的蛋白质组学研究(扩大蛋白质组)。b . Barplots展示肽和蛋白质组的数量后保留过滤那些出现在至少50%的受试者从灌木丛生的或早期非小细胞肺癌。c . Barplots显示不同的数量丰富(DA): 1)蛋白质组,与使用MaxLFQ倒塌的丰度;2)在NPs(即蛋白质组。独立,DA在NPs); and 3) peptides across NPs. D. Volcano plot showing the significance (adjusted p-value; y-axis) and fold change (x-axis) from calculating the differential abundance of protein groups across NPs between healthy and early NSCLC subjects. Protein groups with a log2(Fold Change) greater or less than 1.0 and adjusted p-value < 0.05 are highlighted, where protein groups with increased abundance in early NSCLC subjects are shown in orange and protein groups with increased abundance in healthy subjects are shown in teal. Proteins with known roles in cancer and immune response (ITIH2, CRP, S100A9, S100A8, ANTXR2, and ANTXR1) are highlighted with various shapes. E. Volcano plot showing the significance (adjusted p-value; y-axis) and fold change (x-axis) from calculating the differential abundance of peptides across NPs between healthy and early NSCLC subjects. Peptides with a log2(Fold Change) greater or less than 1.0 and adjusted p-value < 0.05 are highlighted, where peptides with increased abundance in early NSCLC subjects are shown in orange and peptides with increased abundance in healthy subjects are shown in teal. Peptides mapping to proteins with known roles in cancer and immune response (ITIH2, CRP, S100A9, S100A8, ANTXR2, and ANTXR1) are highlighted with various shapes. Credit:《公共科学图书馆•综合》(2023)。DOI: 10.1371 / journal.pone.0282821
