图1:共焦显微镜和CLEM透露的环状结构Httex1 72 q HEK细胞内含物。Httex1抗体的抗原决定基映射用于这项研究。b共焦成像Httex1 72 q夹杂物HEK细胞转染48 h后形成的。所有的计画抗体显示强烈的免疫反应性夹杂物的边缘。细胞核与DAPI和f -肌动蛋白与phalloidin复染色。白色的箭头表示colocalization Httex1 f -肌动蛋白与环状结构的夹杂物。比例尺= 20μm和10μm。c, d CLEM Httex1 72 q夹杂物。c共焦成像Httex1 72 q夹杂物HEK细胞转染48 h后形成的。所选区域(白色广场)被EM (d)检查。正交投影(奥尔特。 Pr.), Scale bars = 5 μm. d The presence of membrane fragments and vesicles are indicated by the white arrowheads. The binary image (inset) shows the core and the shell ultrastructure of the Httex1 72Q inclusion. Scale bar = 500 nm. e Representative EM images of Httex1 72Q inclusion. Higher magnification (white square) are shown in the right panel. Dashed lines delimit the aggregate and the core of the inclusion. Internalized membranous structures and the mitochondria are indicated by the orange and the green arrowheads, respectively. Scale bar = 1 μm and 500 nm. f 3D model of Httex1 72Q cellular inclusion and surrounding organelles (top view). The Httex1 inclusion body (IB) shell is represented in purple, the core in cyan. ER membranes are shown in green, intra-inclusion membranous structures in white, the nucleus in blue, mitochondria in yellow, and the stacked ER cisternae are indicated by a red arrowhead. Scale bar = 2 μm. g Representative EM images of Httex1 39Q inclusion formed 48 h after transfection in HEK cells. The white square indicates the area shown in the right panel at higher magnification. Dashed lines delimit the inclusion. Scale bar = 1 μm and 500 nm. h 3D model of the Httex1 39Q IB is shown in purple, surrounded by mitochondria (yellow), ER structures (green), stacked ER cisternae (red arrowheads), intra-inclusion membranous structures are shown in white and the nucleus in blue. Scale bar = 2 μm. Credit: DOI: 10.1038/s41467-021-26684-z
