HMGB1积极刺激感觉神经元释放的。(A和B) Optogenetic感觉神经元的刺激诱发HMGB1释放。DRG神经元从VGlut2-ChR2-YFP老鼠刺激与控制黄灯(595 nm, n = 3)或蓝色(470 nm, n = 4) 20赫兹,10%的关税周期为30分钟。(A)共焦图像,图像显示了易位HMGB1的细胞质细胞核。固定的感觉神经元与DAPI染色(蓝色),YFP(绿色,glutamatergic神经元中表达),和anti-HMGB1(红色)。插图显示了合并的图像DAPI, YFP, HMGB1染色。注意强胞质易位HMGB1的感觉神经元刺激和蓝光,而神经元刺激控制光展览主要核表达。(酒吧、规模20µm。)数据是代表三个独立的实验。(B)感觉神经元释放HMGB1在激活。蓝色条表明optogenetic刺激的持续时间。 A time-dependent increase in secreted HMGB1 levels is observed following optogenetic stimulation of sensory neurons. Data are represented as mean ± SEM. Two-way ANOVA followed by Sidak’s multiple comparisons test between groups: control vs. blue light stimulation (at 60 min: **P < 0.01, at 90 min: ****P < 0.0001). (C) Optogenetic stimulation does not induce neuronal cell death. Cell viability was measured by LDH release. Cell lysate was included as a positive control for LDH measurements. n = 3 to 4 separate experiments, and each performed in duplicate. Data are represented as individual experimental data point with mean ± SEM. One-way ANOVA followed by Tukey’s multiple comparisons test between groups: ****P < 0.0001. Credit: DOI: 10.1073/pnas.2102034118
